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This is a comparative macrolichen study completed on selected islands in Lake of the Woods near Kenora, Ontario. The study looked at two distinct island habitats, oak savannah, found on the southern edges of the islands, and the prominent boreal coniferous forest. Plots along transects were run on the northern and southern sides of islands for 50 meters. Ground macrolichens were collected and identified using dichotomous keys and thin-layer chromatography. Species richness and lichen frequency were recorded along with the abiotic factors of canopy openness, plot coverage, soil moisture, sunlight amount, soil pH, and tree location. The boreal habitat had a lichen frequency twice as large as in the oak habitat. The species diversity was similar for both the habitat types and the number of unique lichens collected. The species richness was larger for the boreal habitat when compared to the oak habitat. This is the first systematic lichen floral study completed in this area.