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With genetic engineering, systems explored in this book now exist allowing§for the simple, efficient, and near universally precise genetic manipulation§directly in any organism, including the mouse. Herein, these models are applied§to a wide field of disease areas, including diabetes, cardiovascular disease,§skin disorders, cancer, neurodegenerative and neuromuscular diseases, retinal§disorders, as well as various behavioral models. Written for the highly§successful Methods in Molecular Biology§series, chapters include introductions to their respective topics, lists of the§necessary materials and reagents, step-by-step, readily reproducible laboratory§protocols, and tips on troubleshooting and avoiding known pitfalls. § Practical§and fully updated, Mouse Models for Drug§Discovery: Methods and Protocols, Second Edition serves to equip the reader with an extensive overview§of techniques to utilize the many possibilities of mice in the drug development§process.